REAL TIME -PCR FOR QUANTITATIVE DETECTION OF TOXOPLASMA GONDII IN ABORTED WOMEN BEFORE AND AFTER TREATMENT

Huda Al-Marsomy, Elham Alswad, Jabbar Salman Hassan, Haleema Salman

Abstract


Background: Human Toxoplasmosis is a parasitic disease caused by a Toxoplasma gondii. Toxoplasma gondii was the subject of disease burden estimations as a result of the severe clinical symptoms and lifelong implications. Primary infections with T.gondii acquired during pregnancy are usually asymptomatic for the pregnant woman but can lead to serious neonatal complications, isolation of parasite despite it is considered as a golden standard parameter to detect infection, the repetitive B1 gen is the most target gene used in conventional PCR for molecular detection of T.gondii.

Objective: Detection of T. gondii DNA using Real- time PCR seems to be a reliable method in diagnosis and follow up of patients.

Methods: Thirty patients (aborted women), their range age between (20− 46) years, were included in this study. All patients were assessment of Real time Polymerase chain reaction from the same patients was taken before and after spiramycin therapy to confirm the infection of the T.gondii and to evaluate the parasite load (copy number).

Result: Data of  this study revealed that there is significant difference(P=0.002) in the mean of the copy numbers in aborted women's, the mean of parasite copy number in patients samples before treatment was (4.24E+06) and the mean of copy number after treatment was  (7.53E+05).                               

Conclusion: The diagnosis of toxoplasmosis is mainly based on serological tests detection of T. gondii DNA using Real time- PCR seems to be a reliable method in diagnosis and follow up of patients.

Keywords: Toxoplasma gondii, Real- time PCR, B1-gen


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